Past Funding
NIH/NIDDK 5T32DK007467 PI: Shankland 7/15/2014-6/30/2024
Research Training in Renal Disease
The overall goal is to train basic and clinical scientists in kidney-related research so that they will be ideally positioned to
successfully compete for mentored funding, and ultimately transition to research independence. We have developed four
major research training tracks (metabolic disorders and biomarkers; glomerular and tubulointerstitial diseases; clinical
epidemiology and health services research; end-stage renal disease and complications).
NIA 5R01AG046231 PI: Shankland 06/15/2016-12/14/2021
Reduced Glomerular Progenitors Impair Regeneration in Aged Kidney
The need to better understand the effects of aging on the kidney is ever more important with the increasing aging population.
The grant will define how the decline in podocyte number with advancing age cannot adequately be replaced by their
neighboring parietal epithelial cell progenitors, which leads to kidney scarring.
NIDDK UH3DK107343 PI: Shankland, Zheng 09/23/2015-08/31/2020
Rebuilding the glomerular filtration barrier by regenerating adult podocytes (Re)BuildingAKidney.org
Podocytes are cells in the kidney’s glomerular filtering units that limit the passage of proteins from the blood into the urine. As
adults, they cannot proliferate to replace themselves, and therefore they are reliant on other stem cells for their regeneration. In
this grant, we will study such stem cells in podocyte repair to rebuild a kidney.
Department of Defense W81XWH-16-1-0168 PI: Shankland 09/30/2016-09/29/2019
New podocyte-targeted treatments for focal segmental glomerulosclerosis (FSGS)
Our overall goals are to improve drug biodistribution directly to podocytes while decreasing systemicexposure to active drug,
and to demonstrate drug efficacy and safety in an animal model of FSGS. To meet these objectives we propose the following
aims: Aim 1. Optimize and characterize cyclic “sunflower” polymer kidney accumulation in a mouse model of FSGS. The goal of
this aim is to synthesize and optimize a drug delivery vehicle that preferentially distributes to and is retained in the kidney after
injection.
NIH UG3TR002158 Himmelfarb (PI) Shankland (Co-I) (07/25/17-06/30/19)
A Microphysiological System for Kidney Disease Modeling and Drug Efficacy Testing
The goal of this application is to model important human kidney diseases and promote identification of safe and effective treatments. To
achieve this goal, we have established a multidisciplinary investigative team with expertise in kidney physiology and pathology, cellular
and molecular biology, systems pharmacology and toxicology, biomarker discovery and evaluation, biomedical engineering, microfluidics,
matrix biology, genomics, computational biology, and biostatistics.
NIDDK (04/01/2018-03/31/2019)
12th International Podocyte Conference
Conference, with the goal to ultimately reduce the burden of disease in this patient population through improved disease
detection and treatment.
Department of Defense Shankland, Punn (MPI) (06/15/2016-12/14/2018)
Development of cell therapies for FSGS
The goal of this project is to develop a clinically plausible podocyte regeneration therapy using nanoparticle-enhanced autologous uRPCs
(NP-uRPCs).
NIH R01 5 R01 DK093493-02 Shankland (PI) (9/03/2012-6/30/2017)
Pericyte-endothelial cross talk in vascular stability after kidney injury
These studies will investigate the mechanisms by which pericytes nurture kidney blood vessels and the mechanisms by which they detach
in response to injury and thereafter fail to nurture. In understanding these processes we hope to develop new therapies to treat kidney
diseases.
NIH/NIDDK 5 R01 DK083391-01A2 Alpers (PI) Shankland (Co-I) (9/15/2011-8/31/2016)
Podocyte depletion and regeneration during evolution and reversal of diabetic nephropathy
This grant explores mechanisms underlying reversibility (replacement of kidney cell populations that are typically lost in human and
experimental diabetic kidney disease) with emphasis on the podocyte, a unique cell type in the kidney. This grant also focuses on testing
new therapies, based on correcting metabolic injuries to mitochondria (an organelle present within all cells), that are specifically directed
to podocyte mitochondria, as a way to promote reversal of diabetic kidney disease
NIH/NIDDK 5 R01 DK056799 Shankland (PI) (9/1/2011-4/30/2016)
Cell Cycle and Podocytes
The goal of the first aim of this competitive renewal is to show a new paradigm for cell cycle protein function, based on our preliminary
data In summary, the studies proposed will advance scientific knowledge in glomerular diseases, and in cell cycle protein research. The
overall goal of the second aim is to show new regulatory paradigms for cyclin I-p35-cdk5, which play a fundamental role in glomerular and
brain development. In summary, the studies proposed will advance scientific knowledge in glomerular diseases, and in cell cycle protein
research.
NIH – R01- DK083391 Shankland (Co-I) (9/15/2011-8/31/2015)
Podocyte depletion and regeneration during evolution and reversal of diabetic nephropathy
This grant explores mechanisms underlying reversibility (replacement of kidney cell populations that are typically lost in human and
experimental diabetic kidney disease) with emphasis on the podocyte, a unique cell type in the kidney. This grant also focuses on testing
new therapies, based on correcting metabolic injuries to mitochondria (an organelle present within all cells), that are specifically directed
to podocyte mitochondria, as a way to promote reversal of diabetic kidney disease.
NIH Basic Training Grant T32 DL07467-21 Shankland (PI) (09/01/2009–8/31/13)
Nephrology Training 03
This is a training grant that supports research training for three M.D. post-graduate fellows who undergo periods of both renal and basic
science research training.
NIH R24 1R24DK094768 Shankland (PI) (8/01/2012 – 7/31/2014)
Kidney Progenitor Cells in Disease
These initial studies will study progenitor cells in the adult kidney that have the capacity to generate new kidney cells particularly of the
blood cells of the kidney. Investigators will determine how important these progenitors are in regeneration after kidney damage.
Biogen, IDEC MA Inc. Shankland (PI) (4/1/2013-6/31/2014)
Fn14 biology for discovery of new targets that regulate the fate of pericytes
NIH R01 DK-084077 Shankland (PI) (9/1/2009 – 5/31/2014)
The role of macrophage delivered WNT signaling in kidney injury and repair
Determine the role of macrophage delivered Wnt signaling to epithelial cells in kidney repair after ischemic injury, and the role of Wnt
signaling from macrophages to fibroblasts in fibrosis progression.
Boehringer Ingelheim Pharmaceuticals Inc. Shankland (PI) (5/02/2013-05/01/2014)
The Role of CTGF Mouse and Human Pericyte Activation and Disease
American Heart Association 11GRNT7810021 Shankland (PI) (7/1/2011-6/30/13)
Cell Cycle Regulation of Podocyte Proteins
The overall goal of this grant proposal is to prove that both slit diaphragm and actin-binding proteins are Regulated by the cdk5-cyclin I-
p35 complex, and that any alterations in this complex such as occurs in disease, leads to proteinuria and glomerulosclerosis.
NIH/NIDDK 1 R13 DK095622 Shankland (PI) (01/01/2012-12/31/2012)
9th International Podocyte Conference
This R13 grant application is written to support the the 9th International Podocyte Conference, the major goal of which is to substantially
advance the clinical and research aspects of podocyte diseases
NIH-RO1- Renewal Shankland (PI) (7/1/2011 - 6/30/2016)
Cell Cycle and Podocyte Apoptosis
Studies are designed to determine the role of Cyclin I and CDK5, and when active, this complex enhances Survival of terminally differential
cells such as podocytes. The signaling pathways will be delineated. Cell culture and experimental models will be used to delineate this
further.
NIH-RO1 Shankland (PI) (7/1/10 - 6/30/15)
Podocyte Progenitor Cells
The focus of this grant application is to delineate how terminally differentiated podocyte cell number is Restored following injury in
glomerular disease. Based on our recent data and preliminary data, we have compelling evidence that renin-expressing juxtaglomerular
cells and parietal epithelial cells both serve as local progenitor cells. We will show that they both switch their phenotype in disease, and
ultimately become podocytes.
NIH/NIDDK 2R01DK056799 Shankland (PI) (09/01/2011-04/30/2012)
Cell Cycle and Podocytes
The goal of the first aim of this competitive renewal is to show a new paradigm for cell cycle protein function, based on our preliminary
data In summary, the studies proposed will advance scientific knowledge in glomerular diseases, and in cell cycle protein research. The
overall goal of the second aim is to show new regulatory paradigms for cyclin I-p35-cdk5, which play a fundamental role in glomerular and
brain development. In summary, the studies proposed will advance scientific knowledge in glomerular diseases, and in cell cycle protein
research.
NIH ARRA Grant (DK056799-07S1) Shankland (PI) (09/21/09-08/31/11)
Cell Cycle and Podocyte Apoptosis
Studies are designed to determine the role of Cyclin I and CDK5, and when active, this complex enhances survival of terminally differential
cells such as podocytes. The signaling pathways will be delineated. Cell culture and experimental models will be used to delineate this
further.
NIH R01 Grant (DK056799-05A1) Shankland (PI) (09/30/07 – 6/30/11)
Cell Cycle and Podocyte Apoptosis
The overall goal of this grant is to delineate new paradigms in the regulation of podocyte survival and death, so that ultimately new
strategies can be developed to prevent podocyte loss, enhance kidney survival, and reduce kidney disease.
NIH NIDDK ARRA R21 (DK081835-01A2) Shankland (PI) (08/15/09 – 06/30/11)
New Thoughts on Parietal Cells
The precise biological role of parietal epithelial cells (PECs) are not well understood in health or disease. The main goal of this grant is to
show that PECs are required to limit filtered proteins ‘escaping’ into the peri-glomerular space. We will test the hypotheses that the intra-
cellular tight junctions in PECs, together with the underlying Bowman’s Basement membrane, forms a second barrier to proteinuria. We will
also test the hypothesis that PECs actively take up filtered albuminuria which injures them by inducing apoptosis.
1 F32 DK072788-01 Shankland (PI) (9/19/05-12/30/07)
HHS Logar Fellow
DHHS 5901 Letter of Credit (LOC) Shankland (PI) (4/1/2008-3/31/09)
7th Intl Podocyte Conference
This grant will be used to support the travel of junior investigators to attend scientific conference.
American Heart Association – Established Investigator Award 0340129N Shankland (PI) (1/01/03– 12/31/08)
Role of Cell Cycle in Podocyte Injury
The proposed studies are designed to test novel aspects of cell cycle proteins in disease, so that potential therapeutic interventions can be
designed to reduce the incidence of renal failure.
NIH R01 Grant 51096-05 A2 Shankland (PI) (09/01/04-06/30/08)
Cell Cycle Control in Glomerular Disease
This competitive renewal will continue to examine the role of specific cell cycle regulatory proteins in podocyte proliferation and
differentiation.
5 F32DK070434-02 Shankland (PI) (9/12/05-8/30/08)
Immune Renal Injury IA
Tests the central hypothesis that a novel mechanism limiting podocyte proliferation is DNA damage.
NIH/NIDDK R13 Shankland (PI)
6th International Podocyte Conference, Helsinki, Finland
This grant will be used to support the travel of junior investigators to attend this scientific meeting.
American Diabetes Association (7-04-RA-107-80-0572) Shankland (PI) (07/01/04-06/30/06)
Role of Podocytes in Diabetic Nephropathy
The overall goal of this grant is to delineate the mechanisms underlying the abnormalities in podocytes, utilizing cultured cells and animal
models of diabetic nephropathy.
NIH R01 grant DK 60525-02 Shankland (PI) (07/01/02-06/30/06)
Mechanisms of Podocyte Injury
The major goals of this grant are to study the effect of stress-tension-induced injury on podocytes, and also to determine the role of novel
cell cycle regulatory proteins in podocyte disease.
AMGEN – Applied Molecular Genetics 200516848 Shankland (PI) (11/28/05-12/31/07)
Proteinuria Darbepoetin
The goals of this grant are to test the hypotheses that darbepoetin prevents the decrease in nephrin and podocin levels and thereby limits
proteinuria.
AMGEN - Applied Molecular Genetics 200420479 Shankland (PI) (09/27/04-09/26/06)
Role of Erythropeitin (EPO) in Proteinuria
The overall goal of this grant is to test whether a decrease in endogenous EPO in renal disease augments loss of podocytes differentiation,
preventing progressive proteinuria and scarring.
NIH R01 grant DK 56799-04 Shankland (PI) (05/01/00-02/28/05)
Cell Cycle Proteins and Glomerular Apoptosis
The major goal of this project is to show novel roles for specific cell cycle proteins in glomerular cell apoptosis beyond that of proliferation.
NIH/NIDDK R13 DK069139-01 Shankland (PI) (06/01/04-06/30/05)
“5th International Podocyte Conference”
This grant was used to support the travel of junior investigators to attend this scientific meeting.
Juvenile Diabetes Research Foundation (JDRF) 1-2001-395 Shankland (PI) (01/01/01-12/31/03)
The Role of Cyclin Kinase Inhibitors in Diabetic Glomerular Hypertrophy
The major goal of this project will be to determine the nuclear mechanisms underlying the development of diabetic hypertrophy, with a
focus on specific CDK inhibitors.
NIH R01 grant DK 47659-10 Shankland (PI) (09/01/98-08/31/03)
Immunological Renal Diseases: Subproject #2, “The Role of Cell Cycle Proteins in Glomerular Epithelial Cell Growth”
The major goal of this project is to test the hypothesis that specific CDK-inhibitors p57-WT1 determines the proliferative capacity of the
glomerular visceral epithelial cell.
NIH R01 grant DK 51096 Shankland (PI) (06/01/96-05/31/04)
Cell Cycle Control in Glomerular Disease
The major goal of this grant is to determine the expression and activity of specific cell cycle proteins in mesangial cell proliferation in vitro
and in vivo.
NIH Center Grant P50-DK 47659 Shankland (PI) (09/01/98-08/31/03)
George M. O’Brien Kidney Research Center
Subproject #2: The Role of Cell Cycle Proteins in Glomerular Epithelial Cell Growth
The major goal of this project is to test the hypothesis that WT1 determines the proliferative capacity of the glomerular visceral epithelial
cell.
NIH (R01) Grant; DK 52121 Shankland (PI) (01/01/97-12/31/01)
Role of the Glomerular Endothelial Cell in Hemolytic Uremic Syndrome (HUS)
The goal was to determine the mechanisms of endothelial cell proliferation and apoptosis.
C. V. Therapeutics, Inc. Grant Shankland (PI) (01/01/00-12/31/00)
Role of Cell Cycle Proteins in Hemodialysis Vascular Stenosis
The study was designed to determine the role of cell cycle proteins in vascular access stenosis.